HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM. Positive lysates LY400150 (100ug) and LC400150 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and L1CAM-Knockout HeLa cells (KO, Cat# LC832196 ) were separated by SDS-PAGE and immunoblotted with anti-L1CAM monoclonal antibody TA500353 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Anti-L1CAM mouse monoclonal antibody (TA500353) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM (RC211601).
Flow cytometric analysis of living Hela cells, using anti-L1CAM antibody (TA500353, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).
Flow cytometric analysis of living A375 cells, using anti-L1CAM antibody (TA500353, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).
Western blot analysis of extracts (35ug) from 3 cell line lysates by using anti-L1CAM monoclonal antibody (1:500).
HEK293T cells transfected with either RC211601 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody (TA500353), and then analyzed by flow cytometry.