HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM. Positive lysates LY400150 (100ug) and LC400150 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and L1CAM-Knockout HeLa cells (KO, Cat# LC832196 ) were separated by SDS-PAGE and immunoblotted with anti-L1CAM monoclonal antibody TA501437 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Flow cytometric analysis of living A375 cells, using anti-L1CAM antibody (TA501437, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).
Flow cytometric Analysis of Jurkat cells, using anti-L1CAM antibody (TA501437), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Western blot analysis of extracts (35ug) from 3 cell line lysates by using anti-L1CAM monoclonal antibody (1:500).
Anti-L1CAM mouse monoclonal antibody (TA501437) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM (RC211601).
HEK293T cells transfected with either RC211601 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody (TA501437), and then analyzed by flow cytometry.
Flow cytometric analysis of living Hela cells, using anti-L1CAM antibody (TA501437, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).