HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCTD14 (RC200745, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCTD14. Positive lysates LY411435 (100ug) and LC411435 (20ug) can be purchased separately from OriGene.
Anti-KCTD14 mouse monoclonal antibody (TA502636) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KCTD14 (RC200745 ).
Flow cytometric Analysis of Jurkat cells, using anti-KCTD14 antibody (TA502636), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells transfected with either RC200745 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KCTD14 antibody (TA502636), and then analyzed by flow cytometry.