HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCTD14 (RC200745, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCTD14. Positive lysates LY411435 (100ug) and LC411435 (20ug) can be purchased separately from OriGene.
Anti-KCTD14 mouse monoclonal antibody (TA502529) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KCTD14 (RC200745).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-KCTD14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502529)
HEK293T cells transfected with either RC200745 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KCTD14 antibody (TA502529), and then analyzed by flow cytometry.