HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (RC205673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2. Positive lysates LY401961 (100ug) and LC401961 (20ug) can be purchased separately from OriGene.
Anti-IGF2BP2 mouse monoclonal antibody (TA501273) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2 (RC205673).
Western blot analysis of extracts (35ug) from 11 cell line lysates by using anti-IGF2BP2 monoclonal antibody (1:500).
Flow cytometric analysis of living 293T cells transfected with IGF2BP2 overexpression plasmid (RC205673, Red)/empty vector (PS100001, Blue) using anti-IGF2BP2 antibody (TA501273). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).