HVEM/NF-kappaB Reporter Jurkat Recombinant Cell Line

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AMS.79310
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Recombinant clonal stable Jurkat T cell line expressing firefly luciferase gene under the control of 4 copies of NF-kappaB response elements with constitutive expression of human HVEM (Herpes Virus Entry Mediator/ Tumor Necrosis Factor Receptor Superfamily Member 14/ TNFRSF14/ CD270/ GenBank Accession No. NM_003820). Following activation by human HVEM ligand LIGHT/ NF-kappaB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter gene.Materials Required but Not Supplied: Assay Medium: Thaw Medium 2 ( Cat. #60184)LIGHT/ His-Tag (Human) ( Cat. #71266LIGHT-CHO Recombinant Cell Line ( Cat. # 79262)96-well tissue culture-treated white clear-bottom assay plateOne-Step luciferase assay system ( # 60690) or other luciferase reagents for measuring firefly luciferase activityLuminometer
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SKU AMS.79310
Size 2 vials
Species Human
Cell Line JURKAT
Tissue Type Lymphoid Tissue
Application • Screen for agonists or antagonists of LIGHT-HVEM signaling in a physiological relevant cellular context • Characterize T cell-mediated immune responses of HVEM and its interactions with LIGHT • Screen co-stimulatory immune checkpoint molecules
Gene synonyms HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR
Storage Store in liquid nitrogen immediately upon receipt.
Shipping Temp -80C (dry ice)
Supplier Name BPS
Research Areas Immunology
For more details visit our Immunotherapy page >