Immunofluorescent staining of SNB-19 cells using anti-GFAP antibody (TA500341/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).
Western blot analysis of extracts (35ug) from 1 cell line lysate by using anti-GFAP monoclonal antibody (1:500).
Anti-GFAP mouse monoclonal antibody (TA500341) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GFAP (RC204548).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP. Positive lysates LY419563 (100ug) and LC419563 (20ug) can be purchased separately from OriGene.