FOXO Reporter Kit (PI3K/AKT Pathway)
Product Code
60643
$0.00
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The FOXO Reporter kit is designed to monitor activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cultured cells. The kit contains the transfection-ready FOXO3 expression vector and the FOXO luciferase reporter vector|which is a PI3K/Akt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimers of the FOXO responsive element located upstream of a minimal promoter. The FOXO reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector|which serves as an internal control for the transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector also contains the firefly luciferase gene under the control of a minimal promoter|but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
SKU | 60643 |
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Size | 500 reactions |
Alternate SKU | AMS.60643 |
Application | • Monitor PI3K/AKT signaling pathway activity and FOXO transcriptional activity. |
Gene synonyms | FOXO reporter, forkhead reporter, PI3K reporter, AKT reporter, FOXO1 reporter, FOXO3 reporter, FOXO4 reporter |
Format | assay kit |
Storage | Stable at least 12 months from date of receipt# when stored as directed (-20C) |
Shipping Temp | -20C |
Supplier Name | BPS |
Research Areas | Immunology |