Western blot analysis of extracts (10ug) from 1 cell line by using anti-FGFR2 monoclonal antibody at 1:200.
Cross-Reactivity of anti-FGFR2 (TA502877) with FGFR1 and FGFR3, not with FGFR4.HEK293T cells were transfected with the DDK (FLAG)-tagged DNAs of FGFR2 (RC217098), FGFR1 (RC202080), FGFR3 (RC215533), FGFR4 (RC204230) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2 (TA502877). (1:500) and anti-DDK (1:2000), respectively.
Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY FGFR2 (RC217098) using anti-FGFR2 antibody (TA502877/green, left). 293T cells transfected with empty vector served as a negative control (right) (1:100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (RC217098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2. Positive lysates LY400050 (100ug) and LC400050 (20ug) can be purchased separately from OriGene.