Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and MAPK1-Knockout Hela cells (KO, Cat# LC810185) were separated by SDS-PAGE and immunoblotted with anti-MAPK1 monoclonal antibody TA500468. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500468)
HEK293T cells transfected with either RC204703 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK1 antibody (TA500468), and then analyzed by flow cytometry.
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-MAPK1 monoclonal antibody (1:200).
Immunoprecipitation (IP) of MAPK1 by using TrueMab monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAPK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1. Positive lysates LY408481 (100ug) and LC408481 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500468)