HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPM2AIP1 (RC209239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPM2AIP1. Positive lysates LY415014 (100ug) and LC415014 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPM2AIP1 monoclonal antibody.
Anti-EPM2AIP1 mouse monoclonal antibody (TA501793) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EPM2AIP1 (RC209239).
HEK293T cells transfected with either RC209239 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EPM2AIP1 antibody (TA501793), and then analyzed by flow cytometry.