Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and KRT8-Knockout Hela cells (KO, Cat# LC810123) were separated by SDS-PAGE and immunoblotted with anti-KRT8 monoclonal antibody TA500020, (1:2000. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Anti-KRT8 mouse monoclonal antibody (TA500020) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8 (RC209570).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8. Positive lysates LY419425 (100ug) and LC419425 (20ug) can be purchased separately from OriGene.