HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (RC203308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1. Positive lysates LY413042 (100ug) and LC413042 (20ug) can be purchased separately from OriGene.
Anti-CISD1 mouse monoclonal antibody (TA500927) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1 (RC203308).
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CISD1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500927, Dilution 1:50)