HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCOCO2 (RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2 (1:2000).
Flow cytometric analysis of living 293T cells transfected with CALCOCO2 overexpression plasmid (RC203843), Red)/empty vector (PS100001, Blue) using anti-CALCOCO2 antibody (TA502153). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).
Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY CALCOCO2 (RC203843) using anti-CALCOCO2 antibody (TA502153/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).