Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-BTK monoclonal antibody (1:200).
Immunofluorescent staining of HT29 cells using anti-BTK mouse monoclonal antibody (TA501118).
Flow cytometric analysis of living 293T cells transfected with BTK overexpression plasmid (RC211582), Red)/empty vector (PS100001, Blue) using anti-BTK antibody (TA501118). Cells incubated with a non-specific antibody (Green) were used as isotype control.
Figure from citation: Flow Cytometry analysis of intracellular BTK expression by using anti-BTK antibody (clone OTI10E10) in CD14+ monocytes. Black histograms indicate BTK expression and Gray histograms indicate isotype control. BTK expression in the patient was reduced compared with that in a normal control. View Citation
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (RC211582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK. Positive lysates LY424947 (100ug) and LC424947 (20ug) can be purchased separately from OriGene.
Anti-BTK mouse monoclonal antibody (TA501118) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK (RC211582).
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100Â°C for 10min, TA501118, Dilution 1:50)