HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC5 (RC205935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5. Positive lysates LY400469 (100ug) and LC400469 (20ug) can be purchased separately from OriGene.
Flow cytometric analysis of living 293T cells transfected with BIRC5 overexpression plasmid (RC205935), Red)/empty vector (PS100001, Blue) using anti-BIRC5 antibody (TA502236). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).
Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY BIRC5 (RC205935) using anti-BIRC5 antibody (TA502236/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).