HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (RC208947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1. Positive lysates LY419662 (100ug) and LC419662 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTNNB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502299)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.
HEK293T cells transfected with either RC208947 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody (TA502299), and then analyzed by flow cytometry.