HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP. Positive lysates LY400455 (100ug) and LC400455 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501783, Dilution 1:50)
HEK293T cells transfected with either RC206622 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AFP antibody (TA501783), and then analyzed by flow cytometry.