Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACSS2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ACSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503609)
HEK293T cells transfected with either RC204260 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACSS2 antibody (TA503609), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACSS2 (RC204260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACSS2. Positive lysates LY412981 (100ug) and LC412981 (20ug) can be purchased separately from OriGene.