Animal & Endotoxin-free Growth Factors & Cytokines

Increase the Reproducibility of your Stem Cell Research

amsbio is a leading supplier of recombinant proteins and now offers of new range of growth factors and cytokines tailored to stem cell research that are both animal-free and endotoxin-free.

• No contamination from serums, antibiotics, infectious agents
• No unwanted growth factors and cytokine carryover
• FDA G.R.A.S. status (Generally Recognized As Safe)

• Eliminates harmful cellular responses due to LPS contamination

Accumulated data suggests that the endotoxin limits generally allowed in E. coli produced growth factors pose a threat to stem cell research

The success of stem cell research depends on the development of optimal cell culture systems. Both expansion and differentiation of stem cells is highly reliant on growth factor and cytokine supplements, with the activity and purity of these recombinants being critical to successful research and clinical applications. Despite recognised limitations - E. coli and mammalian cells remain the dominant expression systems for commercial recombinant protein production. Although significant improvements in manufacture have been made, both systems present a risk of unwanted contamination from pathogens and endotoxins.

AMSBIO now offers recombinant growth factors and cytokines produced in a plant based expression system utilizes barley seed that cut the risks associated with endotoxin, viral infection and other human pathogen infection.

Endotoxins are an unavoidable by-product in E.coli recombinant protein production and due to their stability and affinity to proteins difficult to completely remove. Currently endotoxin levels in recombinant protein products below 1 EU/mg (<0.1 ng/mg) is accepted as safe limit, however there has long been evidence to suggest the level of endotoxin that will not result in an adverse response varies between cell types, with unwanted effects observed below this accepted limit.

In an embryoid bodies based in vitro stem cell study (1), endotoxin exposure, as low as 0.005 ng/ml, was demonstrated to seriously effect stem cell differentiation by inhibiting mesoderm formation. This concentration correlates to adding growth factors with 0,1 ng/µg (~1 EU/µg) bacterial endotoxins to a stem cell culture.

1. Sivisubramaniyan K, et.al.Endotoxin-induced silencing of mesoderm induction and functional differentiation: role of HMGB1 in pluripotency and infection. Regen Med. 2008 Jan;3(1):23-31

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