Histone Acetylation

From acetyltransferases to deacetylases, we have the tools you need

Acetyltransferases are enzymes that transfer an acetyl group from acetyl CoA to lysine amino acids. In the case of histone acetyltransferases (HATs), these lysine residues reside on histone tails. Generally, addition of acetyl groups to histone tails results in gene activation by inducing a euchromatin conformation and recruiting bromodomain-containing transcription factors for genes in close proximity to the acetylated histone. In a reaction mediated by deacetylase enzymes, such as histone deacetylases (HDACs) and sirtuins, the modification can be removed, thereby allowing for controlled, dynamic regulation of gene expression. Deacetylases, as the name implies, are enzymes that remove acetyl groups from protein substrates. The most well studied deacetylases are histone deacetylases (HDACs). HDACs oppose the effects of histone acetyltransferases (HATs), restoring the positive charge of the histone tail lysine residue. Removal of the acetyl group results in a more tightly compacted, heterochromatin conformation, thereby negatively regulating gene expression.

Deacetylase Assay Kits

NamePacksize Order
HDAC Fluorogenic Assay Kit (Green)96 rxns. View
HDAC1 Fluorogenic Assay Kit96 rxns. View
HDAC1 Kinetic Assay Kit96 rxns. View
HDAC11 Fluorogenic Assay Kit96 rxns. View
HDAC2 Fluorogenic Assay Kit96 rxns. View
HDAC2 Kinetic Assay Kit96 rxns. View
HDAC3 Fluorogenic Assay Kit96 rxns. View
HDAC3 Kinetic Assay Kit96 rxns. View
HDAC4 Fluorogenic Assay Kit96 rxns. View
HDAC5 Fluorogenic Assay Kit96 rxns. View
HDAC7 Fluorogenic Assay Kit96 rxns. View
HDAC8 Fluorogenic Assay Kit96 rxns. View
HDAC9 Fluorogenic Assay Kit96 rxns. View
SIRT1 (Sirtuin1) Fluorogenic Assay Kit96 rxns. View
SIRT2 (Sirtuin2) Fluorogenic Assay Kit32 rxns. View
SIRT3 (Sirtuin3) Fluorogenic Assay Kit32 rxns. View
SIRT5 (Sirtuin5) Fluorogenic Assay Kit96 rxns. View
Sirtuin 6 Fluorogenic Assay Kit96 rxns. View

Deacetylases

NamePacksize Order
HDAC1, FLAG-Tag, His-Tag Recombinant50 µg View
HDAC1, His-Tag, FLAG-Tag (Mouse) Recombinant50 µg View
HDAC10, FLAG-tag Recombinant50 µg View
HDAC11 Recombinant10 µg View
HDAC2, FLAG-tag Recombinant50 µg View
HDAC2, His-tag Recombinant50 µg View
HDAC3/NcoR2, His-Tag, GST-Tag Recombinant50 µg View
HDAC4, GST-tag, His-tag Recombinant10 µg View
HDAC5 (full length), GST-tag Recombinant5 µg View
HDAC5, Catalytic Domain, His-tag Recombinant10 µg View
HDAC5, GST-Tag, His-Tag Recombinant5 µg View
HDAC6 (H216A), GST-Tag Recombinant50 µg View
HDAC6 (H611A), GST-tag Recombinant50 µg View
HDAC6, FLAG-tag Recombinant50 µg View
HDAC6, GST-Tag (HEK293-derived) Recombinant50 µg View
HDAC6, GST-Tag (Mouse) Recombinant50 µg View
HDAC6, GST-tag (Sf9-derived) Recombinant50 µg View
HDAC7, GST-tag Recombinant10 µg View
HDAC7, GST-tag Recombinant25 µg View
HDAC7, GST-tag Recombinant5 µg View
HDAC8, His-Tag Recombinant50 µg View
HDAC9, His-tag Recombinant10 µg View
LpxC (E. coli) Recombinant20 µg View
Sirtuin 1 (193-741), GST-tag Recombinant100 µg View
Sirtuin 2, His-Tag Recombinant100 µg View
Sirtuin 3, GST-Tag Recombinant100 µg View
Sirtuin 4, GST-tag Recombinant100 µg View
Sirtuin 5, GST-tag Recombinant100 µg View
Sirtuin 6, GST-tag Recombinant100 µg View
Sirtuin 7, FLAG-Tag Recombinant100 µg View
pf-HDAC1, Malaria, His-tag, FLAG-tag Recombinant20 µg View

Substrates

NamePacksize Order
Fluorogenic HDAC Substrate 2 (Green fluorescence)250 nmole View
Fluorogenic HDAC class 2a substrate 2 (green)250 nmole View
Fluorogenic HDAC class 2a substrate250 nmole View
Fluorogenic HDAC substrate 3250 nmole View
Fluorogenic HDAC/SIRT substrate 1500 nmole View
Fluorogenic Sirtuin 5 Substrate250 nmole View
Fluorogenic Sirtuin 6 Substrate50 µl View

Acetyltransferase Assay Kits

NamePacksize Order
GCN5 Chemiluminescent Assay Kit96 rxns. View
P300 Chemiluminescent Assay Kit96 rxns. View

Acetyltransferases

NamePacksize Order
GCN5 Chemiluminescent Assay Kit96 rxns. View
P300 Chemiluminescent Assay Kit96 rxns. View