Histone Acetylation

From acetyltransferases to deacetylases, we have the tools you need

Acetyltransferases are enzymes that transfer an acetyl group from acetyl CoA to lysine amino acids. In the case of histone acetyltransferases (HATs), these lysine residues reside on histone tails. Generally, addition of acetyl groups to histone tails results in gene activation by inducing a euchromatin conformation and recruiting bromodomain-containing transcription factors for genes in close proximity to the acetylated histone. In a reaction mediated by deacetylase enzymes, such as histone deacetylases (HDACs) and sirtuins, the modification can be removed, thereby allowing for controlled, dynamic regulation of gene expression. Deacetylases, as the name implies, are enzymes that remove acetyl groups from protein substrates. The most well studied deacetylases are histone deacetylases (HDACs). HDACs oppose the effects of histone acetyltransferases (HATs), restoring the positive charge of the histone tail lysine residue. Removal of the acetyl group results in a more tightly compacted, heterochromatin conformation, thereby negatively regulating gene expression.

Deacetylase Assay Kits

NameDatasheetPacksize Order
HDAC Fluorogenic Assay Kit (Green)-96 reactions View
HDAC Fluorogenic Assay Kit-96 reactions View
HDAC class 2a Fluorogenic Assay Kit96 reactions View
HDAC1 Fluorogenic Assay Kit96 reactions View
HDAC1 Kinetic Assay Kit96 reactions View
HDAC11 Fluorogenic Assay Kit-96 reactions View
HDAC2 Fluorogenic Assay Kit-96 reactions View
HDAC2 Kinetic Assay Kit-96 reactions View
HDAC3 Fluorogenic Assay Kit-96 reactions View
HDAC3 Kinetic Assay Kit-96 reactions View
HDAC4 Fluorogenic Assay Kit-96 reactions View
HDAC5 Fluorogenic Assay Kit-96 reactions View
HDAC6 Fluorogenic Assay Kit96 reactions View
HDAC7 Fluorogenic Assay Kit-96 reactions View
HDAC8 Fluorogenic Assay Kit-96 reactions View
HDAC9 Fluorogenic Assay Kit-96 reactions View
SIRT1 (Sirtuin1) Fluorogenic Assay Kit-96 reactions View
SIRT2 (Sirtuin2) Fluorogenic Assay Kit-32 reactions View
SIRT3 (Sirtuin3) Fluorogenic Assay Kit-32 reactions View
SIRT5 (Sirtuin5) Fluorogenic Assay Kit-96 reactions View
Sirtuin 6 Fluorogenic Assay Kit-96 reactions View

Deacetylases

NameDatasheetPacksize Order
HDAC1, FLAG-tag, His-tag-50 ug View
HDAC1, His-Tag, FLAG-Tag (Mouse)-50 ug View
HDAC10, GST-tag, His-tag-50 ug View
HDAC10, N-FLAG-tag-50 ug View
HDAC11-10 ug View
HDAC11, GST-tag-50 ug View
HDAC2, FLAG-tag-50 ug View
HDAC2, His-tag-50 ug View
HDAC3/NcoR2 Complex, His-tag-50 ug View
HDAC4, GST-tag, His-tag-10 ug View
HDAC5 (full length), GST-tag-5 ug View
HDAC5, Catalytic Domain, His-tag-10 ug View
HDAC5, GST-Tag, His-Tag-5 ug View
HDAC6 (H216A), GST-tag50 ug View
HDAC6 (H611A), GST-tag50 ug View
HDAC6, FLAG-tag-50 ug View
HDAC6, GST-Tag (HEK293-derived)-50 ug View
HDAC6, GST-Tag (Mouse)-50 ug View
HDAC6, GST-tag-50 ug View
HDAC7, GST-tag-10 ug View
HDAC7, GST-tag-25 ug View
HDAC7, GST-tag-5 ug View
HDAC8, His-tag-50 ug View
HDAC9, His-tag-10 ug View
LpxC-20 ug View
NcoR2 (SMRT), GST-tag-50 ug View
Sirtuin 1 (193-741), GST-tag-100 ug View
Sirtuin 2, His-tag-100 ug View
Sirtuin 3, GST-tag-100 ug View
Sirtuin 4, GST-tag-100 ug View
Sirtuin 5, GST-tag-100 ug View
Sirtuin 6, GST-tag-100 ug View
Sirtuin 7, FLAG-tag-100 ug View
pf-HDAC1, Malaria, His-tag, FLAG-tag-20 ug View

Substrates

NamePacksize Order
Fluorogenic Deacetylated substrate Standard250 nmol View
Fluorogenic HDAC Substrate 2 (Green fluorescence)250 nmol View
Fluorogenic HDAC class 2a substrate 2 (green)250 nmol View
Fluorogenic HDAC class 2a substrate250 nmol View
Fluorogenic HDAC substrate 1500 nmol View
Fluorogenic SIRT substrate 1500 nmol View
Fluorogenic Sirtuin 5 Substrate250 nmol View
Fluorogenic Sirtuin 6 Substrate50 ul View

Acetyltransferase Assay Kits

NamePacksize Order
GCN5 Chemiluminescent Assay Kit96 reactions View
P300 Chemiluminescent Assay Kit96 reactions View
P300 Homogeneous Assay Kit384 reactions View

Acetyltransferases

NamePacksize Order
GCN5 Chemiluminescent Assay Kit96 reactions View
P300 Chemiluminescent Assay Kit96 reactions View
P300 Homogeneous Assay Kit384 reactions View