MagSi-DNA cleanFIX kit
All your sequencing sample purifications on any scale in one single solution
AMSBIO offer a one solution approach for DeoxyDye™ terminator removal, enabling researchers to use one kit for any type of sample and volume from
routine sequencing. MagSi-DNA cleanFIX kit addresses the practical need for a fast solution for any lab, any sample volume and any throughput.
Key features of the MagSi-DNA cleanFIX kit:
- Single component kit, saving space in lab and fridge
- Fixed protocols for 5-, 10 and 20 μl sample volumes
- Suitable for single tube, 96- and 384-plate processing
- Consistent quality of processed samples ensured
- Short processing times in any case
- Tailored protocols for individual robotic set-ups possible
- Cost efficient solution in any circumstance
Comparing alternative techniques for DNA sequence sample cleaning, MagSi-DNA cleanFIX kit shows reduced
processing times up to 6 fold
Table 1: Comparison of clean up techniques used for DeoxyDye™ terminator removal
|
|
Processing
Time |
Fully automatable |
MTP plate format |
steps involved |
| MagSi-DNA
cleanFIX kit |
< 25 minutes*
|
yes
|
96, 384, (tube)
|
no centrifugation,
2 washing step |
| Other Bead based
kits |
25 minutes |
yes
|
96, 384, (tube) |
no centrifugation,
2 washing step |
| Column based
kits |
> 15 minutes |
no
|
96, (tube) |
centrifugation and
1 washing step |
| Gel / Sephadex
clean-up |
> 30 minutes |
no
|
no MTP format possible
|
centrifugation and multiple washing steps |
| Ethanol
precipitation |
> 2 hour |
no
|
no MTP format possible |
centrifugation and multiple washing steps |
DNA cycle sequencing reactions
The MagSi-DNA cleanFIX kit is an efficient tool for removal of DyeDeoxy™ terminators from DNA cycle sequencing reactions
using ABI™ terminator kits. Our MagSi-DNA cleanFIX kitsolution enables researchers to process up to 384 samples in one plate. By using the patented MagSi
nanoparticle technology, it offers an excellent opportunity to automate existing processes when dealing with large and growing amounts of samples in the lab.
Figure 2: Data proving the quality of our set-up. Details: 10 μl sequence sample,
template used: pGEM as provided in ABI Big Dye kit V3.0, injection time ABI3730xl sequencing system: 13 seconds. Data kindly provided by Dr Jost Muth, Frauenhofer
IME institute Aachen. Software: ABI Sequence scanner v1.0.
High quality DNA sequencing readout
The MagSi-DNA clean FIX kit is designed to enable high quality DNA sequencing data read outs with the original ABI Sequence scanner v1.0 software.
Readouts from approximately 20-30 bp to > 700 bp can be achieved. The analysis in this case on sample F02_003 generates values of 964 bases, and these CRL-values are considered
'long' in accordance with general accepted views.
Figure 3: Quality control data provided for F02_003 sample as shown in Fig 2.
Quality Control aspects of MagSi-DNA cleanFIX kit
The visual readout indicates the quality of the clean-up of crude sequence samples.
As in the Legend (figure 3), Trace Score values > 20 are set as 'high' in this standard set-up of the software. However many DNA sequencing core
facilities appreciate and adopt much higher Trace Scores (> 40). With MagSi-DNA cleanFIX kit on average the score for sample F02_003 is: 43.
|