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Genea Biocells Skeletal Muscle Differentiation Kit is the world's first kit to differentiate human pluripotent stem cells into functional myotubes. A highly efficient media based protocol to
differentiate skeletal muscle cells from pluripotent stem cells in a simple, scalable fashion.
Benefits:
- Achieves typically 70% MF20-positive myotubes
- Simple 3-step process of media change and cell passaging
- No cell sorting steps required
- No transfection of myogenic transcription factors
- Tested on a wide range of embryonic & induced pluripotent stem cell lines
Generation of Muscle Cells in 3 Steps:
Figure 1. Cultures populations typically comprise of 70-90% muscle myotybes and 10-25% muscle precursor cells.
Cells are phenotypically mature and fully functional.
Figure 2. High content readout for three cell lines differentiated to muscle-committed myoblasts stained with markers MyoD and MyoGenin and mature myotubes stained with the mature muscle markers Dystrophin and MF20.
All kit components are avialable to order separately.
Visit our stem cell research product pages.
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