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Genea Biocells developed the world’s first protocol to differentiate human pluripotent stem cells to skeletal muscle with high yields and without cell sorting or genetic manipulation. Myotubes are contractile,
express typical muscle markers and show striated sarcomeres. The process yields almost pure populations of myogenic cells; it is highly efficient and works robustly with all stem cell lines – critical prerequisites to
use this technology effectively for disease modeling and drug development.
Muscle is generated from human pluripotent stem cells in 3 steps, via satellite-like or progenitor cells and myoblasts that then fuse to multinucleated myotubes in the third step.
This third generation of the process includes an optional fusion enhance that replaces the regular third step.
Benefits:
- Achieves typically 70% MF20-positive myotubes
- Simple 3-step process of media change and cell passaging
- No cell sorting steps required
- No transfection of myogenic transcription factors
- Tested on a wide range of embryonic & induced pluripotent stem cell lines
Generation of Muscle Cells in 3 Steps:
Figure 1. Cultures populations typically comprise of 70-90% muscle myotybes and 10-25% muscle precursor cells.
Cells are phenotypically mature and fully functional.
Figure 2. High content readout for three cell lines differentiated to muscle-committed myoblasts stained with markers MyoD and MyoGenin and mature myotubes stained with the mature muscle markers Dystrophin and MF20.
All kit components are avialable to order separately.
Visit our stem cell research product pages.
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