Extracellular Matrix Heparanase and
Hyaluronidase Activity Kits
AMSBIO supplies Razie assay kits to measure enzyme activity of heparanase
and hyaluronidase in cell culture supernatants, human plasma, biological fluids and tissue samples.
Our kits have been used for research into
kidney disease and
Heparanase and hyaluranidase enzymes both play an important role in the extracellular matrix (ECM).
Our kits provide a solution to the performance and standardization problems found with traditional measurement techniques used for these types of enzymes.
Suitable for inhibitor screening
Fast and easy to use
Sensitive and specific
Use universal 96-well plate format ideal for inhibitor studies
Figure 1: Glycosaminoglycan substrates heparin, heparin sulfate and hyaluronan (hyaluronic acid).
Heparanase Assay Kit
Heparan sulfate proteoglycans (HSPG) are known to play important structural and functional roles in linking the component proteins of the basement membrane, controlling membrane permeability properties and signal transduction.
This latter function is achieved by controlling the bioavailability of cytokines and
growth factors to their cellular receptors.
HSPG on the cell surface participates in signal transduction by facilitating the interaction between growth factors and their receptors.
Heparanase is an endoglycosidase that specifically cleaves heparan sulfate (HS) side chains of HSPG.
Due to the wide distribution and functions of HSPG, heparanase activity affects various cellular functions under normal and pathological conditions.
Research into the development of inhibitors or enhancers of this enzyme had been previously obstructed by the lack of sensitive and specific tests for human heparanase activity.
Furthermore the unavailability of a purified enzyme or instability of the cloned enzyme limits assay design.
To date the available tests have the above shortcomings and are time consuming, not applicable for inhibitor screening or lack an appropriate positive control.
The heparanase assay kits below have been specifically developed to overcome these problems.
Heparanase Assay Workflow
Figure 2: The heparanase assay workflow showing the principles of the assay.
This workflow is the same for the hyaluronidase assay kit, but hyaluronidase treatment occurs instead in the first step and the substrate is hyaluronic acid (HA).
Hyaluronidase Assay Kit
Hyaluronic acid is an important structural macromolecule in the ECM, and involved in the interaction of matrix components.
Mammalian hyaluronidase is an ECM enzyme that degrades hyaluronic acid via hydrolysis of the 1-4 bond between N-acetyl glucosamine and D-glucuronic.
Hyaluronidase can also cleave similar glycosaminoglycans.
Our Razie mammalian hyaluronidase assay kit can be used for screening hyaluronidase inhibitors and for quantification of hyaluronidase activity.
The principles of the kit are very similar to the heparanase assay kit.
Garsen, M., Benner, M., Dijkman, H., van Kuppevelt, T. H., Li, J. P., Rabelink, T. J., ... & van der Vlag, J. (2016).
Heparanase Is Essential for the Development of Acute Experimental Glomerulonephritis.
The American Journal of Pathology
Garsen, M., Sonneveld, R., Rops, A. L., Huntink, S., van Kuppevelt, T. H., Rabelink, T. J., ... & van der Vlag, J. (2015).
Vitamin D attenuates proteinuria by inhibition of heparanase expression in the podocyte
The Journal of pathology, 237(4), 472-481
Martin, L., De Santis, R., Koczera, P., Simons, N., Haase, H., Heinbockel, L., ... & Schuerholz, T. (2015)
The Synthetic Antimicrobial Peptide 19-2.5 Interacts with Heparanase and Heparan Sulfate in Murine and Human Sepsis
PloS one, 10(11), e0143583.
Masola, V., Maran, C., Tassone, E., Zin, A., Rosolen, A., & Onisto, M. (2009).
Heparanase activity in alveolar and embryonal rhabdomyosarcoma: implications for tumor invasion
BMC cancer, 9(1), 1
Masola, V., Gambaro, G., Tibaldi, E., Brunati, A. M., Gastaldello, A., D'Angelo, A., ... & Lupo, A. (2011).
Heparanase and syndecan-1 interplay orchestrates FGF-2-induced epithelial-mesenchymal transition in renal tubular cells
Journal of Biological Chemistry, jbc-M111.
Masola, V., Gambaro, G., Tibaldi, E., Onisto, M., Abaterusso, C., & Lupo, A. (2011).
Regulation of heparanase by albumin and advanced glycation end products in proximal tubular cells
Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1813(8), 1475-1482
Morris, B., & Behzad, F. (2014).
The Effects of Gold and Silver Nanoparticles on an Enzymatic Reaction Between Horseradish Peroxidase and 3, 3', 5, 5'-Tetramethylbenzidine.
Biochemistry & Pharmacology: Open Access, 2014.