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PARP / PARG kits & reagents

amsbio.com - PARP / PARG kits & reagents
amsbio supplies a unique range of Poly-ADP-ribose polymerase (PARP) assay kits, enzymes & antibodies for DNA damage research.
PARP-pathway Download PARP BrochurePARP Assay
Download PARP FactsheetPARP Assay

Poly-ADP-ribose polymerase (PARP) catalyzes the NAD dependent addition of poly-ADP-ribose (PAR) to adjacent nuclear proteins. PARP plays an important role in DNA repair but can also lead to apoptosis by depleting the cellular NAD pool. PARP inhibition has been shown to prevent tissue damage in animal models of myocardial & neuronal ischemia, diabetes, septic shock, & vascular stroke.

PARP Assay Kits measure incorporation of biotinylated PAR onto histone proteins, allowing screening of PARP inhibitors & measurement of PARP activity in cell and tissue extracts. PARG Assay Kits allow screening of PARG inhibitors & measurement of PARG activity in cell extracts.

Assay
Catalogue #		 Application Detection Method Sensitivity Time* (hrs)
Universal PARP**
Colorimetric Kit
4677-096-K 		PARP Inhibitor Screen
IC50 Determination 		Biotin NAD Histone ribosylation 0.01-1 Units PARP 3
Chemiluminescent Kit
4676-096-K
Specific PARP
PARP1, PARP2,
80551, 80552,
PARP3, PARP6
80553, 80556<		 PARP Inhibitor Screen
IC50 Determination		 Biotin NAD
Histone ribosylation 		Sensitive 3.5
PARP/Apoptosis**
Colorimetric Kit
4684-096-K		 PARP Inhibitor Screen PARP Activity in Lysates PAR Ab Histone ribosylation 0.1 - 10 mUnits PARP 500 Cells 3
Chemiluminescent Kit 4685-096-K
HT Fluorescent Homogeneous PARP Inhibition Assay
4690-096-K HT PARP Inhibitor Screen NAD Consumption PARP ribosylation 10% PARP inhibition 1.5
PARP in vivo Pharmacodynamic Assay II**
4520-096-K PARP Inhibition in vivo PAR Levels in Lysates PAR Ab in vivo PARylation 2-1000 pg/ml PAR 6.5
PARG Assay Kits**
Colorimetric Kit
4683-096-K		 PARG Inhibitor Screen Biotin NAD PAR degradation 250 pg PARG 4
Chemiluminescent Kit
4682-096-K

*Actual Times may vary depending upon the number of samples being prepared and assayed.
** Provided with a 96 strip well plate

PAR antibodies are specific for PAR polymers synthesized by a variety of PARP isoforms (PARP-1,-2,-3, tankyrase, vPARP, and others).

PARP/PARG antibodies can be used for analysis of protein levels & cleavage of enzymes in apoptotic cells.

PARP/PARG Enzymes can be used as positive control in the Universal Assay Kits, for detailed mechanistic studies, in Western blot analysis in cell extracts, and for drug discovery applications.

Recombinant PAR Polymer (pADPr) With a chain length of 2 to 300 monomers.

PARP inhibitors:3-Aminobenzamide, 4-Amino-1,8-naphthalimide, 6(5H)-Phenanthridinone, Benzamide

PARP substrates:Biotinylated NAD, activated DNA, 10X activated DNA

To find out more Search for all PARP products or download our PARP presentation.
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