PARP1 Colorimetric Assay Kit

Product Code
AMS.80580
$0.00
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The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependentaddition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric ActivityAssay is the biotinylated substrate. With this kit|only three simple steps are required for PARP1reactions. First|histone proteins are coated on a 96-well plate. Next|the PARP1 biotinylatedsubstrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally|the plateis treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate toproduce color that can then be measured using a UV/Vis spectrophotometer microplate reader.
More Information
SKU AMS.80580
Size 96 reactions
Application Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Gene synonyms poly(ADP-ribose)polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1
Storage Stable at least 6 months from date of receipt# when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Shipping Temp -80C (dry ice)
Supplier Name BPS
For more details visit our PARP Assays page >