Oris™ Pro Cell Migration and Invasion Assays
Monitor cells in real time - no Transwell™ membrane to block analysis
Significantly reduce handling - automation friendly design
Adapt to any system -
Use microscopes, high content screening & high content imaging instruments
Creative design - Use pre-coated assays or apply your own extracelluar matrices
Flexibility - Perform kinetic or endpoint based 2-D cell migration or 3-D cell invasion assays
Monitor Phosphorylation Events - Perform
In-Cell Westerns during cell migration & invasion
Oris™ Pro Cell Migration and Invasion Assays enables the use of automated liquid handling equipment for cell seeding
and allows unrestricted access to cells throughout the experiment. The Oris™ Pro Assay uses a non-toxic biocompatible gel), BCG,
to form a cell-free zone on culture surfaces.
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Oris™ Pro 384
Figure 3: Oris™ Pro Cell Migration Analysis:
HUVECs were seeded onto an Oris™ Pro 384 TC plate and an Oris™ 96 TC plate with Stoppers.
A dose-response titration was performed using the actin polymerization inhibitor, Cytochalasin D. Cells were treated for 16 hours, fixed,
and stained for F-actin. Images were acquired using an inverted microscope and a dose-response curve with IC50 was generated (n=4).
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After seeding cells into the well plate, the BCG dissolves permitting cells to migrate into the well centers (Figure 2:
Assay Schematic). Oris™ assays are available
with tissue culture treated or collagen I coated surfaces. Cells can be stained
and the cells in the Detection Zone can be captured and quantifed in real-time
using microscopes and High Content Screening (HCS)/High Content Imaging (HCI)
instruments. Staining live cells at the beginning of the experiment permits
real-time images to be captured at any time point.
Visit our Oris™ Cell and Invasion Migration Assay Page
Oris™ is a registered trade mark of and manufactured by Platypus Technologies, LLC
BD Pathway™ 855 Bioimaging System is a registered trademark of BD Biosciences
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