Oris Pro Cell Migration and Invasion Assays
Monitor cells in real time - no Transwell membrane to block analysis
Significantly reduce handling - automation friendly design
Adapt to any system -
Use microscopes, high content screening & high content imaging instruments
Creative design - Use pre-coated assays or apply your own extracelluar matrices
Flexibility - Perform kinetic or endpoint based 2-D cell migration or 3-D cell invasion assays
Monitor Phosphorylation Events - Perform
In-Cell Westerns during cell migration & invasion
Oris Pro Cell Migration and Invasion Assays enables the use of automated liquid handling equipment for cell seeding
and allows unrestricted access to cells throughout the experiment. The Oris Pro Assay uses a non-toxic biocompatible gel), BCG,
to form a cell-free zone on culture surfaces.
Oris Pro 384
Figure 3: Oris Pro Cell Migration Analysis:
HUVECs were seeded onto an Oris Pro 384 TC plate and an Oris 96 TC plate with Stoppers.
A dose-response titration was performed using the actin polymerization inhibitor, Cytochalasin D. Cells were treated for 16 hours, fixed,
and stained for F-actin. Images were acquired using an inverted microscope and a dose-response curve with IC50 was generated (n=4).
After seeding cells into the well plate, the BCG dissolves permitting cells to migrate into the well centers (Figure 2:
Assay Schematic). Oris assays are available
with tissue culture treated or collagen I coated surfaces. Cells can be stained
and the cells in the Detection Zone can be captured and quantifed in real-time
using microscopes and High Content Screening (HCS)/High Content Imaging (HCI)
instruments. Staining live cells at the beginning of the experiment permits
real-time images to be captured at any time point.
Visit our Oris Cell and Invasion Migration Assay Page
Oris is a registered trade mark of and manufactured by Platypus Technologies, LLC
BD Pathway 855 Bioimaging System is a registered trademark of BD Biosciences