MAPTrix HyGel™ - 3D Hydrogel cell culture
Innovative & versatile 3D cell culture tool
MAPTrix HyGelTM is a recombinant mussel adhesive protein based, semi-synthetic 3D extracellular matrix that is tailored to mimic specific ECM properties. The MAPTrix HyGelTM hydrogel products are composed of two components: MAPTrixTM ECM, a mussel adhesive protein based extracellular matrix (ECM) mimetic and, MAPTrixTM Link, a multi-arm polyethylene glycol derivative. These fragments together form the 3D hydrogel as shown below.
Use of MAPTrixTM HyGel, which is a recombinant animal free extracellular matrix 3D hydrogel, generates a well-controlled and reproducible extracellular microenvironment for 3D cell culture. Currently existing products, such as extracted and reconstituted basement membrane, have associated with them a diverse array of unknown cues which can be highly disadvantageous, particularly when trying to isolate the effects of specific factors. In addition, these products generally have a variable composition as well as variable mechanical properties. Lot-to-lot inconsistency of reconstituted basement membrane is another problem with many existing basement membrane products which result in significant limitations regarding their end-use.
Features
- Free of animal derived components
- All components of MAPTrix HyGelTM are relatively stable
- Readily able to engineer the elasticity and/or pore size of the hydrogel by adjusting the concentration of MAPTrixTM ECM or MAPTrixTM Linker
- Simply mixing each component can form a hydrogel within two hours, under physiological conditions
- All components are soluble in most buffer solutions
- No cytotoxicity or biocompatibility problems
- Easy to embody a mimetic microenvironment for combinatorial signaling
Benefits
- Ready to use formula to create biochemically-defined hydrogel in situ
- Easy to use, stable under refrigerator conditions for 6 months: no freezing required
- High batch-to-batch reproducibility between samples and vials
- Fully compatible with existing cell culture protocols
| MAPTrix™ HyGel Protein Derivative | Mimetic Motif |
|---|---|
| Angio F (Laminin + Fibronectin) | IKVAV + GRGDSP |
| Angio C (Laminin + Collagen) | IKVAV + GFPGER |
| Fibronectin | RGD |
| GRGDSP | |
| REDV | |
| PHSRN-RGDSP | |
| PHSRN | |
| Laminin | YIGSR |
| IKVAV | |
| KAFDITYVRLKF | |
| RQVFQVAYIIIKA | |
| NRWHSIYITRFG | |
| TWYKIAFQRNRK | |
| RKRLQVQLSIRT | |
| RYVVLPR | |
| RNIAEIIKDI | |
| Collagen I | GLPGER |
| GFPGER | |
| DGEA | |
| Collagen IV | GEFYFDLRLKGDK |
| TAGSCLRKFSTM | |
| TAIPSCPEGTVPLYS | |
| Cadherin | LFSHAVSSNG |
| ADTPPV | |
| LRAHAVDING | |
| Vitronectin | KKQRFRHRNRKGYRSQ |
| FRHRNRKGY | |
| Other | VAEIDGIEL |
| FHRRIKA | |
| TTSWSQCSKS |
Applications
- 3D cell culture for primary or stem cells including HUVEC
- 3D coating for a variety of substrates such as tissue engineering scaffolds
The MAPTrix HyGel ™ matrix can be modularly engineered for designed biochemical characteristics by use of either a single MAPTrix ™ ECM product or a combination of MAPTrix™ ECM products.
Featured Citation
A study of electrochemical biosensor for analysis of three-dimensional (3D) cell culture.
Jeong, S.H. et al (2012) Biosensors and Bioelectronics, 35(1), 128-133.
