DNA-In® Neuro is a new transfection reagent that consistently produces high transfection efficiencies in neurons. Neurons are efficiently transfected with minimal
toxicity to support healthy post-transfected neurons, critical for performing assays on uncompromised transfected cells.
- Higher Transfection Efficiency - Two-fold or greater improvement in efficiency
- Exceptionally Low Toxicity - Maximum post-transfection neuron viability
- Highly Robust Performance - Produces consistent & reproducible results
- Quick & Easy-to-Use - An easy to follow, single-tube protocol
Robust, Easy-to-use, One-tube reagent:
About DNA-In® Neuro
Mature cultures of neurons and other neural cells are extremely valuable for in vitro neurotoxicity studies and screening for agents that can slow, stop, or even reverse the course of neurodegenerative diseases.
However, neurons are among the most difficult cell types to transfect. They are very
sensitive to culture conditions, presenting a particular challenge with regards to efficiencies. In addition to yielding low efficiencies, currently available cationic lipid reagents are often
toxic to the cells, compromising post-transfection experimental results. While some viral mediated gene delivery systems have been shown to produce high efficiencies, they are very labour intensive
and inconvenient for most researchers, along with the inherent danger and risk of provoking an immune response in the cell and/or interfering with the host genome.
DNA-In® Neuro was formulated from a novel chemistry for maximum transfection efficiency in neurons. In side-by-side assays with top
competitor reagents significantly higher transfection efficiencies are consistently observed when DNA-In® Neuro is used to transfect primary cortical, hippocampal or
forebrain neurons. Moreover, with low cytotoxic effect DNA-In® Neuro supports neuronal survival and neurite extension post-transfection.
Figure 1. High GFP Expression in Primary Neurons - Primary rat cortical neurons were transfected with DNA-In® Neuro Transfection Reagent and incubated overnight
in complete culture media. The above images were taken 48-hours post-transfection and show uniform, high GFP expression in healthy cells.
Figure 2. Western blot analysis of the transfected neurons using DNA-In® Neuro - Cortical neurons 5 days in culture were transfected with (1) p35 and (2) p25 (pcDNA3.1 (-) Myc His A) plasmids
in 6 well plates 3ug/well and using 12ul of DNA-In® Neuro transfection reagent. After 48 hrs., western blot analysis was performed using 30ug of cell lysates for each plasmid to show expression.
Data courtesy of Dr. Niranjana Amin, NIH, NINDS.
Superior Transfection Efficiency vs. Leading Competitor:
DNA-In® Neuro is a new transfection reagent that consistently produces high transfection efficiencies in neurons, typically achieving a 2-fold or better improvement in efficiency over the competing
reagents currently available, including Lipofectamine® 2000. Moreover, DNA-In® Neuro enables neurons to be efficiently transfected with minimal toxicity to support healthy post-transfected
neurons, critical for performing assays on uncompromised transfected cells.
Figure 2. (Top,Bottom). DNA-In® Neuro Outperforms Leading Competitor Reagent - DNA-In® Neuro was used to transfect plasmid DNA encoding GFP
into 6-day cultured E18 Primary Rat Cortical Neurons. Transfections were performed in 24-well plates using 1.0-3.0& #956;l of DNA-In® Neuro Reagent.
The above data show DNA-In® Neuro significantly outperforms Lipofectamine® 2000 with near 3-fold improvement in transfection efficiency after 24 hours (Bottom Graph).
Duplicate wells were assayed 24-hrs and 48-hrs after transfection.
Lipofectamine® is a Life Technologies, Inc registered trademark