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GenePORTER® Gold
- Super fast protocol, optimized for same-day transfection
- Effective in a broad range of cell lines
- Ideal for high-throughput applications
- Cost effective - your money will go further
- Unique Advanced Conjugation Enhancement (ACE) technology promotes effective
delivery with exceptionally low cytotoxicity
Rapid
Protocol (click to enlarge)
GenePORTER® Gold Transfection Reagent is our newest, highly effective and reproducible lipid based gene delivery
formulation for the widest variety of cell lines, especially the easy-to-transfect cell lines. It utilizes our Advanced Carrier
Enhancement(ACE) technology that maximizes transfection efficiency and transgene expression levels with minimal cytotoxicity.
The GenePORTER® Gold Reagent and protocol have been optimized to provide the fastest, easiest, and most cost effective transfection
reactions possible. Because of its simplicity and cost advantage, the GenePORTER® Gold Transfection Reagent is ideal for high
throughput or large scale applications where the need for cost control and great results are highly desired.
GenePORTER® Gold Performance
HEK 293 Figure 1.
Transfection efficiency in HEK 293 cells. HEK 293 cells were plated on poly-D-lysine
coated glass cover slips and transfected with 1ug of gWiz GFP and GenePORTER GOLD,
jetPRIME(Polyplus-transfection) or Lipofectamine 2000(Invitrogen, Inc.) in 12 well plates according to the
manufacturer's recommended protocols. Cells were incubated for 48 hours then permeablized, and
incubated with AlexaFluor® 555 phalloidin (Invitrogen, Inc.), which stains cellular actin red. Cells were
mounted on slides with Prolong® Gold antifade reagent with DAPI (Invitrogen, Inc.), which stains the cell
nuclei blue.Cells were visualized by fluorescence microscopy using identical exposure times for FITC,
TRITC, and DAPI. Transfection efficiency, cell viability and cell morphology are simultaneously assessed
indicating both high transfection efficiency and significantly higher viability for GenePORTER® GOLD
transfected cells relative to cells transfected with jetPRIME and Lipofectamine 2000.
RAW 264.7 Figure 1.
Transfection efficiency in hard-to-transfect RAW 264.7 cells. RAW 264.7 cells
were plated on glass cover slips and transfected with 1ug of gWiz GFP and GenePORTER® GOLD,
jetPRIME (Polyplus-transfection) or Lipofectamine 2000(Invitrogen, Inc.) in 12 well plates according to the
manufacturer's recommended protocols. Cells were incubated for 48 hours then fixed, permeablized, and
incubated with AlexaFluor 555 phalloidin (Invitrogen,Inc.), which stains cellular actin red. Cells were mounted
on slides with Prolong Gold antifade reagent with DAPI (Invitrogen,Inc.), which stains the cell nuclei blue.
Cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI.
Transfection efficiency, cell viability and cell morphology are simultaneously assessed indicating both
high efficiency and significantly higher viability for GenePORTER® GOLD transfected cells relative to cells
transfected with jetPRIME and Lipofectamine 2000.
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