GenePORTER® 3000 Transfection Reagent

High transfection efficiency in hard-to-transfect cells

GenePORTER® 3000 Transfection Reagent is a molecular delivery lipid which can effectively overcome numerous barriers to successful transfection, including cell-specific extracellular membranes, cytoplasmic degradation and the nuclear membrane, while minimizing cytotoxicity.

The key to GenePORTER® 3000’s exceptional ability to transfect difficult cell lines is Advanced Conjugation Enhancement (ACE) technology, a nuclear targeting mechanism that consistently delivers the most effective performance of any reagent on the market.

Features

Designed specifically for hard-to-transfect cells
Minimal cytotoxicity
Optimized for same-day transfection
Can be used with a broad range of cells
Superior performance in both serum & serum-free media

Benefits

Rapid protocol: 4.5 hours total processing time
Higher density cells/wells increases overall expression
Use of freshly detached cells and serum-free protocol improves transfection results noticeably
Amenable to high-throughput applications

Name	Datasheet	Packsize	Order
GenePORTER 3000 Diluent 5 x 4 ml/5 vials	-	5 x 4 ml vials	View
GenePORTER 3000 Transfection Reagent		0.75 ml	View
GenePORTER 3000 Transfection Reagent	-	1.5 ml	View
GenePORTER 3000 Transfection Reagent	-	15 ml	View
GenePORTER 3000 Transfection Reagent Sample	-	25 x 10 reactions	View
GenePORTER 3000 Transfection Reagent, Trial Size		1 x 10 reactions	View

Transfection Efficiency

Different cell lines

20X

60X

Consistent expression results with GenePORTER^® 3000 Transfection Reagent. Cells were transfected with GenePORTER^® 3000 Transfection Reagent and gWiz-GFP Expression Vector DNA 30 minutes after plating. Whole field microscope images were taken from a single slide for different cell types. Cells were visualized 48 hours post transfection and images taken at both a 20X magnification, to see overall transfection efficiency, and at 60X magnification to determine post-transfection cellular health and morphology. The results indicate excellent transfection efficiency, robust GFP expression, and healthy cell morphology.

GenePORTER® vs. Lipofectamine®

GenePORTER® 3000 dramatically improves lipid-mediated transfection into both HepG2 and RAW264.7 cells, eliminating the need to utilize alternative methods of gene delivery such as viral infection or electroporation.

Hep G2 ONPG assay results

RAW 264.7 ONPG assay results

Transfection efficiency of β-galactosidase encoding plasmid into both HepG2 and RAW 264.7 cells is greatly improved when using GenePORTER® 3000 compared to Lipofectamine® 2000.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.

Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.

Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.

Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.

Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.

Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.

Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.

We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.

We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.

We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.

We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.

GenePORTER® 3000 Transfection Reagent

High transfection efficiency in hard-to-transfect cells

Features

Benefits

Transfection Efficiency

Different cell lines

20X

60X

GenePORTER® vs. Lipofectamine®

Hep G2 ONPG assay results

RAW 264.7 ONPG assay results

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GenePORTER® Gold Transfection Reagent

GeneSilencer® siRNA Transfection Reagent

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