GenePORTER® 3000 Transfection Reagent
High transfection efficiency in hard-to-transfect cells
GenePORTER® 3000 Transfection Reagent is a molecular delivery lipid which can effectively overcome numerous barriers to successful transfection, including cell-specific extracellular membranes, cytoplasmic degradation and the nuclear membrane, while minimizing cytotoxicity.
The key to GenePORTER® 3000’s exceptional ability to transfect difficult cell lines is Advanced Conjugation Enhancement (ACE) technology, a nuclear targeting mechanism that consistently delivers the most effective performance of any reagent on the market.
Features
- Designed specifically for hard-to-transfect cells
- Minimal cytotoxicity
- Optimized for same-day transfection
- Can be used with a broad range of cells
- Superior performance in both serum & serum-free media
Benefits
- Rapid protocol: 4.5 hours total processing time
- Higher density cells/wells increases overall expression
- Use of freshly detached cells and serum-free protocol improves transfection results noticeably
- Amenable to high-throughput applications
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| GenePORTER 3000 Diluent 5 x 4 ml/5 vials | - | 5 x 4 ml vials | View |
| GenePORTER 3000 Transfection Reagent | - | 0.75 ml | View |
| GenePORTER 3000 Transfection Reagent | - | 1.5 ml | View |
| GenePORTER 3000 Transfection Reagent | - | 15 ml | View |
| GenePORTER 3000 Transfection Reagent Sample | - | 25 x 10 reactions | View |
| GenePORTER 3000 Transfection Reagent, Trial Size |  | 1 x 10 reactions | View |
20X
60X
Consistent expression results with GenePORTER® 3000 Transfection Reagent. Cells were transfected with GenePORTER® 3000 Transfection Reagent and gWiz-GFP Expression Vector DNA 30 minutes after plating. Whole field microscope images were taken from a single slide for different cell types. Cells were visualized 48 hours post transfection and images taken at both a 20X magnification, to see overall transfection efficiency, and at 60X magnification to determine post-transfection cellular health and morphology. The results indicate excellent transfection efficiency, robust GFP expression, and healthy cell morphology.
Hep G2 ONPG assay results
RAW 264.7 ONPG assay results
