In the desire to make the
search for new drugs more economically viable, pharmaceutical companies are
seeking rapid, sensitive assays where all the reaction reagents are added in a
single automated step. Homogeneous assays are popular with researchers involved
in high-throughput drug screening because they save time, save reagents, and are
easy to automate. Homogeneous assays allow a simple mix and read
process, without requiring lengthy, time consuming wash steps to remove unbound
AlphaScreen® kits are
bead-based ALPHA* technology assays, using microparticles coated with either
DNA or protein in a format suitable for use with automated reagent handling
systems. Each AlphaScreen® or AlphaLISA® assay contains two bead types, Donor
beads and Acceptor beads. Binding of molecules captured on the beads leads to an
energy transfer from one bead to the other, ultimately producing a luminescent /
fluorescent signal. This signal can be detected in a plate reader capable of
reading 96-, 384- or 1596-well plates. There are two types of beads, one used
for AlphaScreen® kits and one for AlphaLISA® kits. The AlphaLISA® bead emission (615 nm)
is narrower and brighter than the AlphaScreen® bead emission (520-620 nm).
Conveniently, amsbio's kits are suitable with either assay format.
© 2007-present, PerkinElmer, Inc. All rights reserved. Reproduced with permission.
These are the first in a
series of kits that broaden the range Kinase PDE and HDAC screening and
profiling products and services available, visit our enzyme activity page
for more information
stands for Amplified Luminescent Proximity Homogeneous Assay. The ALPHA
technology is exclusively offered by PerkinElmer, Inc., and is similar to TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) technology.
AlphaLISA and AlphaScreen are trademarks of