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SoluBL21™ Competent E. coli
- Optimized E. coli strain for expressing proteins in soluble form
- Simple and straightforward protocol
- Compatible with all T7 promoter-based expression vectors
Description
SoluBL21™** Competent E. coli, a significantly improved BL21 host strain for soluble
protein expression. Using a directed evolution approach, a mutant strain of BL21(DE3) E. coli has been developed that
can produce soluble protein in the majority of cases where expression in the parent BL21(DE3) yielded no detectable soluble product.
With SoluBL21™ a major obstacle to effective protein expression in E. coli has been overcome for many mammalian and bacterial proteins.
This significant improvement should enable scientists to make progress in a wide range of applications quicker and far less expensively than in the past.
Latest Publications:
Deatherage C (2012) Purification and Characterization of the Human γ-Secretase
Activating Protein. Biochemistry. Jun 8.
New Downloads
SoluBL21™ Chemically Competent Cells - White Paper 
SoluBL21™ Electrocompetent Cells - White Paper
Expression of Insoluble Proteins in SoluBL21™ vs. standard BL21(DE3) E. coli
Figure 1. A panel of 22 clones that produced little or no soluble protein in standard BL21(DE3) cells were induced side-by-side in BL21(DE3)
and SoluBL21™ E. coli. These clones encoded for a wide range of proteins including: cell cycle proteins, ATP-binding proteins, transferases, isomerases,
kinases, and repair enzymes from human and other mammalian sources. With the SoluBL21 strain, ~70% of previously insoluble proteins were expressed in soluble form.
Examples of SoluBL21-expressed proteins are shown above.
| C700200 (Link to protocol) |
Quantity |
| SoluBL21™ Chemically Competent E. coli |
10 x 50 ul |
| SOC Medium |
5.0 ml |
| pUC19 Positive Control Plasmid |
20 ul (500 pg/ul) |
| C700210 (Link to protocol)
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| SoluBL21™ Electrocompetent E. coli |
10 x 20 ul |
| SOC Medium |
5.0 ml |
| pUC19 Positive Control Plasmid |
20 ul (10 pg/ul) |
Shipping
The SoluBL21™ Chemically Competent E. coli are shipped frozen. For maximum stability and long-term use, store the cells
and pUC19 control plasmid at -70oC upon receipt. All components are guaranteed stable for 6 months when stored properly.
** Patent Pending
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