Trilencer-27 - Guaranteed knockdown for siRNA gene silencing
Genomewide coverage against human, mouse and rat
Trilencer-27 siRNA kit contains Dicer-Substrate duplexes that provide
two critical improvements over the use of traditional 21mer siRNA
designs. 27mer siRNA takes advantage of the natural processing by
Dicer producing 10-fold higher potency and specificity than shorter
21mer RNAi forms. 27mer dicer-substrate duplexes also evade the radar of
the mammalian interferon response when expressed in mammalian cells and
initiates strong and specific gene silencing. By its optimal design,
Trilencer-27 siRNA has the advantages of improved efficacy and minimal interferon response.
Features & Benefits:
- Genomewide coverage against human, mouse and rat
- 27mer Dicer-substrate duplex--higher potency & minimal interferon response
- Guaranteed* gene knockdown (≥70%)
- 3 gene-specific siRNAs + 1 negative control
Search our siRNA Product Range
here.
* We guarantee that at least two of the three Dicer-Substrate duplexes in the
kit will provide at least 70% or more knockdown of the target mRNA when used at
10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent
transfection control duplex (cat# SR30002) indicates that >90% of the cells have
been transfected and the HPRT positive control (cat# SR30003) provides 90%
knockdown efficiency.
For non-conforming siRNA, requests for replacement
product must be made within ninety (90) days from the date of delivery of the
siRNA kit. To arrange for a free replacement with newly designed duplexes,
please contact Technical Services at info@amsbio.com. Please provide your data
indicating the transfection efficiency and measurement of gene expression
knockdown compared to the scrambled siRNA control (quantitative RT-PCR data
required).