Western blot analysis of YBX1 (arrow) using rabbit polyclonal YBX1 Antibody (C-term) (Cat. #TA324830). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2) (Origene Technologies).
YBX1 Antibody (C-term) (Cat. #TA324830) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the YBX1 antibody detected the YBX1 protein (arrow).
IF image of U251 cell stained with YBX1 Antibody (C-term) (Cat#TA324830).U251 cells were incubated with YBX1 primary antibody (1:25, 1 h at 37??C). For secondary antibody, Alexa Fluor?½ 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor?½ 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). YBX1 immunoreactivity is localized to Cytoplasm significantly.