Flow Cytometry - YAP1 antibody; Overlay histogram showing HeLa cells stained with TA300956 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Western blot - YAP1 antibody [EP1674Y];.developed using the ECL technique.Predicted band size : 65 kDa.Observed band size : 70 kDa.Exposure time : 1 minute
Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y]; Immunohistochemical staining of YAP1 in paraffin embedded human breast carcinoma tissue using TA300956 at a 1/100 dilution.
Western blot - YAP1 antibody [EP1674Y]; Anti-YAP1 antibody [EP1674Y] at 1/50000 dilution + Hela cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 65 kDa.Observed band size : 65 kDa.