Anti-ERCC4 mouse monoclonal antibody (TA503294) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC4 (RC223300).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4. Positive lysates LY401605 (100ug) and LC401605 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-ERCC4 monoclonal antibody at 1:200 dilution.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503294)
HEK293T cells transfected with either RC223300 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERCC4 antibody (TA503294), and then analyzed by flow cytometry.