HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC4 (RC223300, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC4. Positive lysates LY401605 (100ug) and LC401605 (20ug) can be purchased separately from OriGene.
Anti-ERCC4 mouse monoclonal antibody (TA503324) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC4 (RC223300).
HEK293T cells transfected with either RC223300 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERCC4 antibody (TA503324), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC4 monoclonal antibody.