HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WTAP (RC223046, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WTAP. Positive lysates LY407186 (100ug) and LC407186 (20ug) can be purchased separately from OriGene.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: WTAP Antibody
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.