Vesicular somatis virus (VSV) tools
Virus-based tools for cell engineering
AMSBIO offers recombinant VSV with the deletion of glycoprotein VSV-G which can accept envelope proteins from a wide range of viruses (pseudoviruses) such as spike protein from SARS-CoV-2. Our VSV delta G pseudotypes are also available in formats expressing reporter genes such as Firefly Luciferaseor Green Fluorescent Protein (GFP) so that viral entry can be monitored using luminescence or fluorescence.
See all VSV products
Advantages of the VSV Delta G model
- Undergoes only one round of replication; therefore, it is safe to use and requires only a biosafety level 2 (BSL-2) facility
- Can be pseudotyped with a variety of viral envelope glycoproteins
- Represents a robust model of viral infection
- High titer
- Infection and viral entry monitored by transduction of luciferase or fluorescent proteins
- SARS-CoV-2 Spike VSV Delta G is the preferred pseudovirus for infection of Vero-6 and Calu-3 cells
Applications
- Study mechanism of infection
- Screen for neutralizing antibodies and other inhibitors of virus entry
How does VSV Delta G Pseudotyping work?
VSV (Vesicular stomatitis virus) is an enveloped, negative-stranded RNA virus with a simple structure. Its ability to infect a wide range of mammalian cell types has made VSV a valuable tool to study virus entry, replication, and assembly. The glycoprotein of VSV (termed VSV-G), which binds to the LDL-receptor (low-density lipoprotein receptor), is responsible for the attachment and entry of VSV into a susceptible host cell.
The VSV-G glycoprotein can be deleted from the VSV (VSV Delta G or VSV ΔG) and replaced with recombinant envelope proteins from a wide variety of viruses, allowing for the generation of pseudotypes that represent robust models to screen for neutralizing antibodies and other inhibitors of virus entry. A VSV Delta G pseudovirus can be engineered to transduce a reporter gene encoding Firefly Luciferase or Fluorescent proteins, so that viral entry can be monitored using luminescence or fluorescence, respectively.
The bald VSV Delta G particles do not contain an envelope glycoprotein and cannot infect cells. They are used as a negative control for transduction.
The Spike Pseudotyped VSV Delta G viral particles contain the trimeric SARS-CoV-2 Spike protein as the envelope protein, and, like SARS-CoV-2, can bind to and infect ACE2-expressing cells.
Coronavirus VSV products
VSV-G has been deleted (VSV Delta G or VSV ΔG) and replaced with a trimeric wild-type or variant SARS-CoV-2 spike protein, generating pseudotypes that represent robust models to screen for neutralizing antibodies and other inhibitors of virus entry. Our VSV Delta G pseudoviruses transduce luciferase so that viral entry can be monitored using luminescence. Safe to use, they require only a Biosafety Level 2 (BSL-2) facility.
SARS-CoV-2 Spike VSV Delta G represents a robust model to study the mechanisms of SARS-CoV-2 interaction with ACE2 (Angiotensin converting enzyme 2), to characterize neutralizing antibodies, and to screen or titrate drugs that prevent viral entry into human cells. It is the preferred pseudovirus to study SARS-CoV-2 infection of Vero-6 and Calu-3 cells.
Product Information Table
| Name | Packsize | Order |
|---|---|---|
| Spike (B.1.617.2/ Delta Variant) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 100 ul | View |
| Spike (B.1.617.2/ Delta Variant) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 500 ul x 2 | View |
| Spike (BA.2.12.1/ Omicron Variant) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 100 ul | View |
| Spike (BA.2.12.1/ Omicron Variant) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 500 ul x 2 | View |
| Spike (BA.2/ Omicron Variant) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 100 ul | View |
| Spike (BA.2/ Omicron Variant) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 500 ul x 2 | View |
| Spike (BA.4/5/ Omicron Variant) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 100 ul | View |
| Spike (BA.4/5/ Omicron Variant) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 500 ul x 2 | View |
| Spike (D614G) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 100 ul | View |
| Spike (D614G) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 500 ul x 2 | View |
| Spike (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 100 ul | View |
| Spike (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) | 500 ul x 2 | View |
| VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) | 100 ul | View |
| VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) | 500 ul x 2 | View |
