HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSIG2 (RC200170, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSIG2. Positive lysates LY415368 (100ug) and LC415368 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VSIG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502161)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VSIG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502161)
HEK293T cells transfected with either RC200170 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VSIG2 antibody (TA502161), and then analyzed by flow cytometry.