Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SLC18A2 monoclonal antibody (1:200).
HEK293T cells transfected with either RC221342 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLC18A2 antibody (TA500506), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SLC18A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500506)
Anti-SLC18A2 mouse monoclonal antibody (TA500506) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC18A2 (RC221342).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC18A2 (RC221342, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC18A2. Positive lysates LY401066 (100ug) and LC401066 (20ug) can be purchased separately from OriGene.
Figure from citation: Western Blot of SLC18A2 protein level by using anti-SLC18A2 (9E11) antibody in the striatum of autopsied human brain. View Citation