Immunohistochemistry (Paraffin-embedded sections) - VEGF antibody [EP1176Y]; Ab52917 (1:50) staining human VEGF in human hemangioma tissue by immunohistochemistry using paraffin embedded tissue.
IHC - Anti-VEGF antibody; TA303731 stainingVEGF in Mouse kidney tissue sections by IHC (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100and blocked with 5% serum for45 minutes at 25¶øC. Samples were incubated with primary antibody (1/400 in4% BSA + 5% serum in PBST) for 14 hours at 4¶øC. An Alexa Fluor? 546-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
Flow Cytometry - VEGF antibody- Carboxyterminal end; Overlay histogram showing HeLa cells stained with TA303731 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells ) used under the same conditions. This antibody gave a positive signal in HeLa cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.