Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and VASP-Knockout HeLa cells (KO, Cat# LC832038) were separated by SDS-PAGE and immunoblotted with anti-VASP monoclonal antibody TA502473 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Anti-VASP mouse monoclonal antibody (TA502473) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP (RC203544).
HEK293T cells transfected with either RC203544 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VASP antibody (TA502473), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (RC203544, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VASP. Positive lysates LY401150 (100ug) and LC401150 (20ug) can be purchased separately from OriGene.