IF image of U251 cells stained with UVRAG (C-term) antibody. U251 cells were treated with Chloroquine, then incubated with TA324649 UVRAG (C-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). UVRAG immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Immunofluorescence staining of Autophagy UVRAG antibody (Cat# TA324649) on Methanol-fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki, Finland.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.