IF image of SK-BR-3 cell stained with USF1 Antibody (Center) (Cat#TA324374). SK-BR-3 cells were incubated with USF1 primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).USF1 immunoreactivity is localized to nucleus significantly.
USF1 Antibody (Center) (Cat. #TA324374) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
USF1 Antibody (Center) (Cat. #TA324374) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow).