Ubiquitination and Deubiquitination

Assay kits and enzymes for ubiquitination and deubiquitination

Ubiquitin is an 8.5 kDa protein that is expressed in almost all tissues, hence the name. Ubiquitination is a post-translational modification in which one or more ubiquitin proteins is added to a substrate protein by a reaction that is carried out in three main steps; activation, conjugation, and ligation. Addition requires the sequential action of 3 enzymes, ubiquitin activating enzymes (E1), ubiquitin-conjugating enzymes (E2), and ubiquitin ligases (E3). Ubiquitination was originally described as the process that labels proteins for degradation by the proteasome, but was later found to have a number of distinct functions such as altered protein localization, modulation of protein-protein interaction, and activation or inactivation of target proteins. In addition to the highly studied proteosomal degradation pathway, ubiquitin modifications have been linked to the regulation of a number of other important cellular processes, including inflammation, response to stress or viral infections, DNA repair, and stem cell differentiation.

Many proteins regulated by ubiquitin modification control cellular processes that are essential for tumorigenesis and tumor growth, including cell cycle progression, apoptosis, and receptor trafficking and down-regulation. Recent years have seen significant progress in our understanding of the molecular basis of ubiquitin modifications in cancer progression and it has therefore become an attractive drug target.

Assay Kits

NamePacksize Order
MDM2 TR-FRET Assay Kit384 reactions View
UBCH13 TR-FRET Assay Kit384 reactions View
USP7 Inhibitor Screening Assay Kit96 reactions View

Activating Enzymes - E1

NamePacksize Order
UBA6 (UBE1L2), FLAG-tag100 ug View
Ubiquitin, His-Tag2 mg View

Conjugating Enzymes - E2

NamePacksize Order
UEV1A (UBE2V1), FLAG-tag100 ug View
UbcH13 (UBE2N), His-tag100 ug View
UbcH5a (UBE2D1), FLAG-tag100 ug View
UbcH5c, His-Tag (Human)100 ug View
UbcH6 (UBE2E1), His-tag100 ug View
UbcH7, His-tag (E. coli-derived)100 ug View
UbcH7, His-tag (Sf9-derived)50 ug View

Ligases - E3

NamePacksize Order
CBL, GST-Tag100 ug View
CBL-B TR-FRET Assay Kit384 reactions View
CBL-B, GST-Tag (Human)100 ug View
CBL-B, His-Avi-Tag (Human)100 ug View
CBLC, FLAG-Tag100 ug View
CUL2, FLAG-Tag20 ug View
CUL3/Rbx1, GST-tag20 ug View
Elob, FLAG-Tag20 ug View
KCTD13, GST-tag20 ug View
NEDD4, FLAG-tag20 ug View
SMURF1, FLAG-tag20 ug View
SMURF2, FLAG-tag20 ug View
UBE3A, His-FLAG-tags20 ug View
UFL1, GST-tag20 ug View
WWP1, FLAG-tag20 ug View
c-CBL TR-FRET Assay Kit384 reactions View