Anti-TUBA3E mouse monoclonal antibody (TA501595) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBA3E (RC209279).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA3E (RC209279, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA3E. Positive lysates LY404091 (100ug) and LC404091 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC209279 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TUBA3E antibody (TA501595), and then analyzed by flow cytometry.