Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TTLL12 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
HEK293T cells transfected with either pCMV6-ENTRY TTLL12 (RC200841) (Red) or empty vector control plasmid (Blue) were immunostained with anti-TTLL12 mouse monoclonal (TA500775, Dilution 1:1,000), and then analyzed by flow cytometry.
Flow cytometric analysis of Jurkat cells, using anti-TTLL12 antibody (TA500775), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTLL12 (RC200841, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12. Positive lysates LY402417 (100ug) and LC402417 (20ug) can be purchased separately from OriGene.
Anti-TTLL12 mouse monoclonal antibody (TA500775) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TTLL12 (RC200841).
Flow cytometric analysis of Hela cells, using anti-TTLL12 antibody (TA500775), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).