HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTC32 (RC209334, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTC32. Positive lysates LY423407 (100ug) and LC423407 (20ug) can be purchased separately from OriGene.
Anti-TTC32 mouse monoclonal antibody (TA501340) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TTC32 (RC209334).
HEK293T cells transfected with either RC209334 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TTC32 antibody (TA501340), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TTC32 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501340, Dilution 1:50)